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Test ID: Beta Hydroxybutyrate
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Beta Hydroxybutyrate
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Useful For
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Aid in diagnosis of diabetic ketoacidosis.
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Method name and description
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UV Method. A kinetic enzymatic method to measure the level of D-3-hydroxybutyrate in serum or plasma. The method is based on the oxidation of D-3-hydroxybutyrate to acetoacetate by the enzyme 3-Hydroxybutyrate dehydrogenase. Concomitant with this oxidation the cofactor NAD+ is reduced to NADH and the associated change of absorbance can be directly correlated with the D-3-hydroxybutyrate concentration.
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Clinical information
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β-Hydroxybutyric acid, also known as 3-hydroxybutyric acid, is an organic compound and a beta hydroxy acid with the formula CH3CH(OH)CH2CO2H; its conjugate base is beta-hydroxybutyrate, also known as 3-hydroxybutyrate.
In diabetic ketoacidosis (DKA), β-hydroxybutyrate is present in the greatest concentration and accounts for approximately 75% of the three ketone bodies. During periods of ketosis, β-hydroxybutyrate increases even more than acetoacetate and acetone and has been shown to be a better indicator of ketoacidosis, including subclinical ketosis.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Li‑heparin tube
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Collection instructions / Special Precautions / Timing of collection
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Collect blood by standard venipuncture techniques as per specimen requirements. When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer.
Preferably patient on fasting
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Storage and transport instructions
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Storage: 7 days at 2 – 8°C;
3 months at ‑20 °C
Transport: 2-8°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample
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Biological reference intervals and clinical decision values
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Plasma fasting level: 0.03 – 0.3 mmol/L
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
STAT: 1 hour
Routine: One working day
Specimen Retention: 4 days
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