|
Test ID: Theophylline Level
|
|
Theophylline Level
|
|
|
|
Useful For
|
Assessing therapeutic and toxic drug level of theophylline.
|
|
Method name and description
|
The assay is based on the kinetic interaction of microparticles in a solution (KIMS). Theophylline antibody is covalently coupled to microparticles and the drug derivative is linked to a macromolecule. The kinetic interaction of microparticles in solutions is induced by binding of drug‑conjugate to the antibody on the microparticles and is inhibited by the presence of theophylline in the sample. A competitive reaction takes place between the drug conjugate and theophylline in the serum sample for binding to the theophylline antibody on the microparticles. The resulting kinetic interaction of microparticles is indirectly proportional to the amount of drug present in the sample.
|
|
Clinical information
|
Theophylline (1,3-dimethylxanthine), a bronchodilator, is widely used to treat patients with asthma, apnea (temporary asphyxia), and other obstructive lung diseases.
Monitoring of theophylline concentrations in serum is essential, since individuals can vary in their rates of theophylline clearance, and severe toxicity has been observed without prior occurrence of minor side effects.Also, monitoring serum theophylline levels may provide the physician with useful information to aid in adjusting patient dosage to achieve optimal therapeutic effect while avoiding drug toxicity.
|
|
Aliases
|
Theophylline, Aminophylline
|
|
|
Specimen type / Specimen volume / Specimen container
|
Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Li‑heparin tube
|
|
Collection instructions / Special Precautions / Timing of collection
|
Collect blood by standard venipuncture techniques as per specimen requirements. When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer
|
|
Storage and transport instructions
|
Storage: 1 week at 2 – 8°C
60 days at ‑20°C
Transport: 2-8°C
|
|
Specimen Rejection Criteria
|
Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample
|
|
|
Biological reference intervals and clinical decision values
|
Therapeutic ranges:
Adult: 56 -111 umol/L
Neonatal Apnea: 33 - 61 umol/L
Pediatric Asthma: 44 - 111 umol/L
|
|
Turnaround time / Days and times test performed / Specimen retention time
|
Daily (24/7)
Turn-around time:
STAT: 1 hour
Routine: One working day
Specimen Retention: 4 days
|
|
|
