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Test ID: Urine Calcium Creatinine Ratio
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Urine Calcium Creatinine Ratio
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Useful For
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Identification of abnormal states causing excess or suppressed excretion of calcium.
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Method name and description
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Calcium ions react with 5-nito-5’-methyl-BAPTA (NM-BAPTA) under alkaline conditions to form a complex. This complex reacts in the second step with EDTA. The change in absorbance is directly proportional to the calcium concentration and is measured photometrically.
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Clinical information
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Calcium is the most abundant mineral element in the body with about 99 % in the bones primarily as hydroxyapatite. The remaining calcium is distributed between the various tissues and the extracellular fluids where it performs a vital role for many life sustaining processes. Among the extra skeletal functions of calcium are involvement in blood coagulation, neuromuscular conduction, excitability of skeletal and cardiac muscle, enzyme activation, and the preservation of cell membrane integrity and permeability.
Increased urinary excretion of calcium accompanies hyperparathyroidism, vitamin D intoxication and following calcium supplementation.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Random Urine (Spot Urine)
Minimum volume of 5 mL freshly voided urine in clean container or aliquoted tube.
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Collection instructions / Special Precautions / Timing of collection
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Urine specimens is recommended to be stored at 2‑8°C when delay in transport.
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Storage and transport instructions
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Storage: 2 days at 15 – 25°C
4 days at 2 – 8°C;
3 weeks at ‑15 to -25°C
Transport: 2-25°C
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Specimen Rejection Criteria
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Leaking specimen, contaminated sample, adulterated sample.
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Biological reference intervals and clinical decision values
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0 - 0.6 mmol/mol creatinine
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
Routine: One working day
Specimen Retention: 4 days
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