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Test ID: Uric Acid
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Uric Acid
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Useful For
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Diagnosis and treatment of numerous renal and metabolic disorders.
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Method name and description
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Enzymatic colorimetric test.
In the presence of peroxidase, 4‑aminophenazone is oxidized by hydrogen peroxide to a quinone‑diimine dye. The color intensity of the quinone‑diimine formed is directly proportional to the uric acid concentration and is determined by measuring the increase in absorbance.
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Clinical information
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Uric acid is the final product of purine metabolism in the human organism. Uric acid measurements are used in the diagnosis and treatment of numerous renal and metabolic disorders, including renal failure, gout, leukemia, psoriasis, starvation or other wasting conditions, and of patients receiving cytotoxic drugs.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Li‑heparin tube
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Collection instructions / Special Precautions / Timing of collection
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Collect blood by standard venipuncture techniques as per specimen requirements. When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer.
EDTA plasma values are approximately 7 % lower than serum values.
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Storage and transport instructions
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Storage: 3 days at 20 – 25°C
7 days at 4 – 8°C;
6 months at ‑20 °C (± 5 °C)
Transport: 4-25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient samples.
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Biological reference intervals and clinical decision values
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Sex
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Age
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Reference Interval (umol/L)
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From
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To
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From
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To
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Male
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0 minutes
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15 days
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158
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748
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Male
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15 days
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1 year
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88
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370
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Male
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1 year
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12 years
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100
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282
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Male
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12 years
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16 years
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150
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446
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Male
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16 years
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150 years
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200
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430
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Female
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0 minutes
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15 days
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158
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748
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Female
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15 days
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1 year
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88
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370
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Female
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1 year
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12 years
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100
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282
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Female
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12 years
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16 years
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147
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342
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Female
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16 years
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150 years
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140
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360
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Factors affecting test performance and result interpretation
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Calcium dobesilate causes artificially low uric acid results. Dicynone (Etamsylate) at therapeutic concentrations may lead to false‑low results Acetaminophen intoxications are frequently treated with N‑Acetylcysteine. N‑Acetylcysteine at the therapeutic concentration when used as an antidote and the Acetaminophen metabolite N‑acetyl‑p‑benzoquinone imine (NAPQI) independently may cause falsely low results.
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
STAT: 1 hour
Routine: One working day
Specimen Retention: 4 days
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