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Test ID: Urea 24 Hour Urine
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Urea 24 Hour Urine
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Useful For
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Diagnosis and treatment of certain renal and metabolic disorders.
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Method name and description
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Kinetic test with urease and glutamate dehydrogenase.
Urea is hydrolyzed by urease to form ammonium and carbonate. In the second reaction 2-oxoglutarate reacts with ammonium in the presence of glutamate dehydrogenase (GLDH) and the coenzyme NADH to produce L-glutamate. In this reaction two moles of NADH are oxidized to NAD+ for each mole of urea hydrolyzed.The rate of decrease in the NADH concentration is directly proportional to the urea concentration in the specimen and is measured photometrically.
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Clinical information
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Urea is the major end product of protein nitrogen metabolism. It is synthesized by the urea cycle in the liver from ammonia which is produced by amino acid deamination. Urea is excreted mostly by the kidneys but minimal amounts are also excreted in sweat and degraded in the intestines by bacterial action.
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Specimen type / Specimen volume / Specimen container
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Specimen type: 24 hour Urine (random specimen is not applicable)
Specimen Container: 24-hour urine container
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Collection instructions / Special Precautions / Timing of collection
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Refer to Specimen Management Procedures
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Storage and transport instructions
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Storage: 2 days at 15 – 25°C
7 days at 2 – 8°C
1 months at ‑15 °C to ‑25 °C
Transport: 2-25°C
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Specimen Rejection Criteria
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Leaking specimen, contaminated sample, adulterated sample.
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Biological reference intervals and clinical decision values
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
Routine: One working day
Specimen Retention: 4 days
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