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Test ID: Measles IgG Antibody
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Measles IgG Antibody
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Detection of Measels IgG Antibody in Blood
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Useful For
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Intended to be used as an aid in the determination of serological status to measles virus.
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Method name and description
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Indirect sandwich chemiluminescence immunoassay (CLIA)
In the presence of complimentary antigen and antibody, the antibody binds to the antigen to form an antigen-antibody or an immune complex.The light signal, the amount of isoluminol-antibody conjugate, is measured by a photomultiplier as relative light units (RLU) and is indicative of analyte concentration present in calibrators, samples or controls.
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Clinical information
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Prior to vaccine availability, measles was mostly a disease of childhood, but measles vaccination programs (part of measles, mumps, rubella [MMR] vaccination) have had a marked effect on the incidence of the disease and the complications associated with it. Both IgM and IgG antibodies are synthesized during the primary immune response and can be detected in the serum within a few days of rash onset. IgM antibody levels peak after about seven to ten days and then decline rapidly, being rarely detectable after six to eight weeks. IgM is generally not detected in an immune individual following re-exposure to measles virus.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
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Collection instructions / Special Precautions / Timing of collection
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Collect blood by standard venipuncture techniques as per specimen requirements. When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer
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Storage and transport instructions
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Storage: 9 days at 2 – 8°C;
Transport: 2-25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient samples.
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Biological reference intervals and clinical decision values
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Analyte/Unit
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Result message
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Result message
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Interpretation/ further steps
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AU/mL
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< 13.5
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Negative
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Negative for Measles IgG, no further testing needed.
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AU/mL
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≥ 13.5 to < 16.5
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Equivocal
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Equivocal samples should be verified as equivocal & comment will be added automatically by system.
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AU/mL
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≥ 16.5
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Positive
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Positive for Measles IgG, no further testing
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
Routine: One working day
Specimen Retention: 4 days
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