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Test ID: EBV Capsid Antigen IgG
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EBV Capsid Antigen IgG
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Detection of Epstein-Barr virus IgG Antibody in Blood
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Useful For
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Assist in diagnosis of Epstein-Barr viral infection. Individual levels of specific antibodies are not necessarily indicative of disease state but can be of diagnostic significance when tracked as an antibody response profile. Antibody response profiles for the different EBV antigens demonstrate a characteristic pattern for silent primary or persistent latent EBV infections, as well as for each of the EBV-associated diseases.
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Method name and description
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Indirect sandwich chemiluminescence immunoassay (CLIA)
In the presence of complimentary antigen and antibody, the antibody binds to the antigen to form an antigen-antibody or an immune complex.The light signal, the amount of isoluminol-antibody conjugate, is measured by a photomultiplier as relative light units (RLU) and is indicative of analyte concentration present in calibrators, samples or controls.
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Clinical information
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Epstein-Barr virus (EBV) is the etiologic agent of infectious mononucleosis (IM). The major route of transmission of EBV is through oral contact. Replication of EBV occurs in the oropharyngeal epithelium and results in the release of virions from infected B lymphocytes, with consequent shedding of infectious particles into the saliva. During childhood, primary infection with EBV is often asymptomatic. Acquisition of the virus during adolescence through adulthood results in infectious mononucleosis in the majority of persons. After primary infection, EBV remains latent for life.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Lithium heparin tube
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Collection instructions / Special Precautions / Timing of collection
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Collect blood by standard venipuncture techniques as per specimen requirements. When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer
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Storage and transport instructions
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Storage: 7 days at 2 – 8°C;
Transport: 2-25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient samples.
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Biological reference intervals and clinical decision values
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Analyte/Unit
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Result message
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Result message
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Interpretation/ further steps
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U/mL
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< 20
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Negative
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Negative for EBV VCA IgG Ab, no further testing needed.
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U/mL
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≥ 20
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Positive
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Positive for EBV VCA IgG Ab
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
Routine: One working day
Specimen Retention: 4 days
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