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Test ID: Rubella Antibody IgG
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Rubella Antibody IgG
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Detection of Rubella IgG Antibody in Blood
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Useful For
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Aid in the determination of the immune status to Rubella and the diagnosis of acute infection.
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Method name and description
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Electrochemiluminescence immunoassay (ECLIA): Sandwich principle
Measurement of antigen or antibody based on the change in electrochemiluminescence (ECL) signal before and after immunoreaction. Results are determined automatically by the software by comparing the electrochemiluminescence signal obtained from the reaction product of the sample with the signal of the cutoff value previously obtained by calibration.
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Clinical information
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Rubella virus is the etiological agent of German measles, a commonly mild rash disease which occurs usually during childhood. However, Rubella can be a serious disease when a pregnant woman becomes infected especially during the first trimester of pregnancy.
Rubella virus can be transmitted through the placenta and results in fetal death or causes severe malformations to the fetus, commonly summarized as congenital Rubella syndrome (CRS). Today, infant vaccination programs and the vaccination of women in childbearing age who are susceptible to Rubella infection have considerably reduced the incidence of acute Rubella infection. The presence of IgG antibodies to Rubella virus indicates a previous exposure either by vaccination or prior Rubella infection and is indicative of immunity.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Li‑heparin tube
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Collection instructions / Special Precautions / Timing of collection
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Collect blood by standard venipuncture techniques as per specimen requirements. When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer
Specimens should not be altered subsequently with additives (biocides, antioxidants or substances possibly changing the pH of the sample) in order to avoid erroneous findings.
Pooled samples and other artificial material may have different effects on different assays and thus may lead to discrepant findings
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Storage and transport instructions
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Storage: 7 days at 20 – 25°C
2 weeks at 2 – 8°C;
3 months at ‑20 °C (± 5 °C)
Transport: 2 – 25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample and heat‑inactivated samples.
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Biological reference intervals and clinical decision values
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Analyte/Unit
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Result message
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Result message
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Interpretation/
further steps
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IU/mL
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< 10 IU/mL
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Non-reactive
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Negative for anti‑Rubella IgG.
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IU/mL
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≥ 10 IU/mL
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Reactive
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Positive for anti‑Rubella IgG. *
The presence of IgG antibodies to Rubella virus is an indication of previous exposure either by prior infection or by vaccination.
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Factors affecting test performance and result interpretation
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In rare cases, interference due to extremely high titers of antibodies to analyte‑specific antibodies, streptavidin or ruthenium can occur. These effects are minimized by suitable test design
Increasing amounts of unspecific human IgG may lead to a decrease in the recovery of positive samples with the Elecsys Rubella IgG assay.
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
Routine: One working day
Specimen Retention: 4 days
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