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Test ID: Haemophilus influenza type b (Hib) AB
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Haemophilus influenza type b (Hib) AB
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Useful For
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Assessment of an antibody response to the Haemophilus influenza type b (Hib) vaccine present in human serum ,This assay is measurement of specific IgG antibodies against Haemophilus influenza type b (Hib) and determine protective status.The test used to aid diagnosis only, A positive result must be confirmed by clinical findings and other serological tests. The results obtained from this assay are not diagnostic proof of disease.
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Method name and description
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Method: Enzyme-Linked Immunosorbent Assay (ELISA)
To perform an ELISA for the detection of Haemophilus influenza type b (Hib) antibodies in patient samples, the microwells are pre coated with Hib antigen. Then, calibrators, controls, and diluted patient samples are added to the wells, and during the first incubation, antibodies that recognize the Hib antigen bind to the wells. After washing the wells to remove unbound proteins, anti-human IgG conjugate is added, which binds to the captured human antibody. Any excess unbound conjugate is removed by another wash step. The bound conjugate is detected with a substrate that generates a blue reaction product, and the intensity of this product is proportional to the antibody concentration in the sample. To stop the reaction, phosphoric acid is added to each well, resulting in a yellow endpoint color that can be read at 450nm.
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Reporting name
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Anti Haemophilus Flu Type B
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Clinical information
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Hib is a Gram-negative bacterium that is encapsulated and can lead to a variety of conditions such as meningitis, septicaemia, cellulitis, epiglottitis, pneumonia, and septic arthritis. It is particularly common in children under the age of five but can also affect adults with specific medical conditions, where it is a significant cause of bacterial meningitis.
The Hib vaccine may be recommended for older children or adults who have had their spleen damaged or removed, including those with sickle cell disease, before undergoing spleen removal surgery, or following a bone marrow transplant. Additionally, people between the ages of 5 and 18 with HIV may be advised to receive the Hib vaccine.
If a patient experiences repeated bacterial infections, it may be necessary to investigate whether they have an immunodeficiency or an inability to respond to specific polysaccharide antigens. To determine a patient's specific antibody response, their IgG anti-Hib antibody levels can be evaluated through serological testing.
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Aliases
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Anti-Haemophilus influenza type B (Hib)
HaemoFlu B
H. influenzae Antibody
Haemophilus influenzae B Vaccine Response
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum sample.
Specimen Volume: At minimum 3 mL whole blood or 0.5 mL serum.
Container Tube: Plan tube /Serum gel (Yellow top tubes).
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Storage and transport instructions
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For sample transportation, the sample should remain at room temperature (18-25°C ) for no longer than eight hours.
Separated serum samples can be stored at 2-8°C for up to 48 hours.
Separated serum samples should be frozen at below -20°C for any long-term storage.
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Specimen Rejection Criteria
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Quantity not sufficient (QNS)
Hemolysis
Lipemic
Wrong collection container
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Biological reference intervals and clinical decision values
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| Interpretation of Test Results |
Reference Values (mg/L) |
| Positive (Vaccinated) |
≥0.15 |
| Negative (Unvaccinated) |
< 0.15 |
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Turnaround time / Days and times test performed / Specimen retention time
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Turnaround Time: 14 working days.
Days and Times test performed: Once every two weeks from 7:00 to 15:00.
Specimen retention time: Approximately one month.
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