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Test ID: Vancomycin
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Vancomycin
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Useful For
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To measure and monitor the concentration of vancomycin in blood.
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Method name and description
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Kinetic interaction of microparticles in solution (KIMS)
Kinetic interaction of microparticles in solution (KIMS) as measured by changes in light transmission on the Roche cobas c-systems. Vancomycin antibody is covalently coupled to microparticles and the drug derivative is linked to a macromolecule. The kinetic interaction of microparticles in solutions is induced by binding of drug‑conjugate to the antibody on the microparticles and is inhibited by the presence of vancomycin in the sample. A competitive reaction takes place between the drug conjugate and vancomycin in the serum sample for binding to the vancomycin antibody on the microparticles. The resulting kinetic interaction of microparticles is indirectly proportional to the amount of drug present in the sample.
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Clinical information
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Vancomycin is a complex glycopeptide antibiotic, which is used for the treatment of infections caused by Gram‑positive organisms, primarily methicillin resistant Staphylococcus aureus (MRSA), coagulase‑negative Staphylococci, Streptococci or Enterococci, particularly in patients allergic to β‑lactams.Monitoring of trough serum or plasma levels is necessary to ascertain clinical efficacy and to limit potentially dose‑dependent serious side effects, e.g. ototoxicity and nephrotoxicity. Meanwhile the relevance of monitoring peak concentrations is questioned by some clinicians due to limited clinical data. (Roche Cobas TDM Vancomycin Gen.3 package insert ; Roche Diagnostic Corp., Indianapolis, IN)
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Li‑heparin tube
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Collection instructions / Special Precautions / Timing of collection
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The time of blood collection must be indicated:
Trough: Just prior to next dose
Peak (not recommended): 1-2 hour after the end of infusion
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Storage and transport instructions
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Storage: 48 hours at 15 – 25°C
14 days at 2 – 8°C
12 months at ‑20 °C
Transport: 2-25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample.
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Biological reference intervals and clinical decision values
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Interpretative Data:
Trough range: 15- 20 ug/ml as per the Infectious Disease Society of America (IDSA) Guidelines
Peak range: Monitoring of peak Vancomycin concentration is not recommended. Peak range 20-40 ug/mL
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
STAT: 1 hour
Routine: One working day
Specimen Retention: 4 days
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