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Test ID: IIF C3
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Indirect Immunofluorescence C3
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Indirect Immunofluoresence Stain
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Useful For
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Demonstration of human C3 in tissues and assessment of pattern, extent and intensity of glomerular staining. Assess presence and pattern of C3 deposits in other sites (Tubular basement membrane, blood vessels, interstitium)
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Method name and description
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- Polyclonal Rabbit C3c Complement/FITC
- Immunofluorescence stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Reporting name
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C3 Indirect Immunofluorescence
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Clinical information
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C3c FITC reacts with human C3c complement and with the C3c part of C3 and C3b. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins. Purified immunoglobulin fraction of rabbit antiserum conjugated with fluorescein isothiocyanate isomer 1. It is essential to supplement clinical findings and histopathology in the diagnosis of autoimmune diseases. It permits early diagnosis, treatment, and subsequent monitoring of disease activity in patients with these potentially life-threatening disorders.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (2 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test: (Manual Staining) 3 hours
- Days and times test performed: Daily (Sunday to Thursday)
- Time: 0700H-1200H
- Note: Request received after 1200H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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