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Test ID: Ferritin
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Ferritin
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Useful For
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Iron deficiency and iron overload conditions diagnosis aid.
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Method name and description
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Electrochemiluminescence immunoassay (ECLIA): Sandwich principle
Electrochemiluminescence immunoassay (ECLIA) intended for use on the cobas e 801 immunoassay analyzer. Patient specimen, biotinylated monoclonal Ferritin‑specific antibodies, and a monoclonal Ferritin‑specific antibody labeled with a ruthenium complex react to form a sandwich complex. After addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell II M. Application of a voltage to the electrode then induces chemiluminescent emission which is measured by a photomultiplier. Results are determined via a calibration curve which is instrument specifically generated by 2‑point calibration and a master curve
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Clinical information
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Ferritin, known as iron-storage protein. The amount of ferritin in serum is an indicator of the iron reserves.
Elevated levels can indicate hemochromatosis, while decreased serum ferritin concentrations always indicate iron deficiency that might be the result of prior blood loss, altered iron uptake, transferrin deficiency, or increased demand (e.g. pregnancy), alcoholic or viral hepatitis and chronic renal failure where its unrelated to iron stores status.
As an acute phase reactant, elevated serum ferritin values can occur in patients with infections, acute or chronic inflammation and malignant tumors, regardless of acute iron deficiency.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum, Plasma
Minimum volume of sample: 1 mL
Serum: Plain tube (red or yellow top)
Plasma: Li‑heparin tube
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Collection instructions / Special Precautions / Timing of collection
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Collect blood by standard venipuncture techniques as per specimen requirements. When processing samples in primary tubes (sample collection systems), follow the instructions of the tube manufacturer.
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Storage and transport instructions
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Storage: 48 hours at 20 – 25°C
7 days at 2 – 8°C;
12 months at ‑20 °C (± 5 °C)
Transport: 2-25°C
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Specimen Rejection Criteria
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Grossly hemolyzed, icteric and lipemic samples, wrong collection container, insufficient sample and heat‑inactivated samples.
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Biological reference intervals and clinical decision values
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Age
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Reference interval (ng/mL or μg/L)
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From
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To
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Male
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Female
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0 Minutes
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6 Months
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6-400
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6-430
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6 Months
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3 years
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12-57
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12-60
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3 years
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14 years
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14-80
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12-73
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14 years
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19 years
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20-155
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12-90
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19 years
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29 years
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38-270
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12-114
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29 years
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39 years
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48-420
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12-160
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39 years
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49 years
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30-490
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12-240
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> 50 years
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30-553
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18-340
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Turnaround time / Days and times test performed / Specimen retention time
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Daily (24/7)
Turn-around time:
Routine: One working day
Specimen Retention: 4 days
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