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Test ID: IIF IgG
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Indirect Immunofluorescence IgG
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Indirect Immunofluoresence Stain
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Useful For
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Demonstration of human IgG in tissues and assessment of pattern, extent and intensity of glomerular staining. Assess presence and pattern of IgG deposits in other sites (Tubular basement membrane, blood vessels, interstitium)
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Method name and description
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- Polyclonal Rabbit Anti-Human IgG/FITC.
- Immunofluorescence stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Reporting name
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IgG Indirect Immunofluorescence
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Clinical information
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IgG FITC reacts with the g-chains of human IgG. Traces of contaminating antibodies have been removed by solid-phase absorption. Polyclonal Rabbit Anti-Human IgG conjugate produced from a purified immunoglobulin fraction of rabbit antiserum. It is essential to supplement clinical findings and histopathology in the diagnosis of autoimmune diseases. It permits early diagnosis, treatment, and subsequent monitoring of disease activity in patients with these potentially life-threatening disorders.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (2 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test: (Manual Staining) 3 hours
- Days and times test performed: Daily (Sunday to Thursday)
- Time: 0700H-1200H
- Note: Request received after 1200H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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