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Test ID: ISH EBER
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EBER RNA CISH
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Immunohistochemistry Stain
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Useful For
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In situ detection of the abundantly expressed EBV-encoded RNA (EBER) genes, can identify lymphoma-associated viral infections, knowledge that can be used to help guide disease management. EBER RNA CISH is intended for the detection of Epstein-Barr Virus (EBV) RNA.
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Method name and description
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- EBER RNA CISH
- Chromogenic In-Situ Hybridization (CISH) stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Clinical information
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It is an oligonucleotide-based probe that binds to the EBER1 and EBER2 nuclear RNAs. The probe is conjugated with fluorescein isothiocyanate (FITC) to enable detection using the Anti-FITC-AP CISH.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide. One slide will be stained for ISH EBER and the other slide will be stained for the ISH +ve probe (the use of the ISH +ve probe is for evaluation of RNA quality by chromogenic in situ hybridization (CISH)) (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 4-5 hours
- Days and times test performed: Once a week (0600H- 1400H)
- Note: Request received after the staining day will be stained the following week.
- Shelf-life of the paraffin section slides: ≤6 weeks
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