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Test ID: Marginal Zone Panel by FISH
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Marginal Zone Panel by FISH
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Marginal Zone Lymphoma (MZL) by FISH test.
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Method name and description
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Interphase Fluorescence In Situ Hybridization (I-FISH) study.
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Clinical information
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Marginal Zone Lymphoma (MZL) includes two lymphoid neoplasms: an extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma) and nodal MZL; the previous is more common than the last mentioned and affects the GI tract, while MZL affects peripheral lymph nodes predominately.
FISH test is outlined by specific target probes that detect the most significant abnormalities in MZL. Swerdlow et al. 2008). FISH studies complement the chromosome analysis, particularly when analyzing the abnormal clone pertaining to relapse and progression, or when the specimen quantity is insufficient for chromosomal analysis.
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Specimen type / Specimen volume / Specimen container
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1. Specimen Type: Blood/Leukemic Blood
Container/Tube: Green top (sodium heparin)
Specimen Volume: 2-6 mL (Varies depending on the WBC-Count).
(Adult - 3-6 mL ,Child - 2 mL, Infant - 2 mL)
2. Specimen Type: Bone marrow
Container/Tube: Bone Marrow Transport Media
Specimen Volume: 1-3 mL (Varies depending on the WBC-Count).
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Collection instructions / Special Precautions / Timing of collection
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Sunday-Wednesday 7:00-14:00
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Storage and transport instructions
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Specimen Rejection Criteria
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Consistent with Diagnostic Genomic Division policies.
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Factors affecting test performance and result interpretation
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Marginal zone lymphomas (MZL) are a type of B cell neoplasms with variable clinical traits. The clinicopathologic entities incorporate Extra Nodal Marginal Zone MALT lymphoma, Nodal Marginal Zone Lymphoma (MZL), and splenic marginal zone lymphoma (MZL).
The MZL FISH panel includes:
1) IGH/MALT1 - t(14;18)
2) IGH -(14q32)
3) BCL6 - (3q27)
4) IGH/CCND1 -t(11;14)
5) BIRC3/MALT1 (aka API2/MALT1) - t(11;18).
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Turnaround time / Days and times test performed / Specimen retention time
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