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Test ID: IH IMP3
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IMP3
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Immunohistochemistry Stain
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Useful For
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Distinguishes between pancreatic ductal adenocarcinoma from chronic sclerosing pancreatitis. IMP3 expression has also been found in a variety of other human cancers including renal cell carcinoma, adenocarcinoma of the uterine cervix, endometrial carcinoma, adenocarcinoma of the esophagus, malignant melanoma, Merkel cell carcinoma, urothelial carcinoma, neuroendocrine carcinoma of the lung, adenocarcinoma of the pancreas, and triple negative breast cancer. Also expressed in Hodgkin’s lymphoma.
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Method name and description
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- Rabbit Monoclonal Antibody IMP3 (EP286)
- Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Clinical information
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Insulin-like growth factor II mRNA binding protein 3 (IMP3) is an oncofetal RNA-binding protein that regulates targets such as insulin-like growth factor-2 and beta-actin. IMP3 expression is at its highest during embryogenesis and silenced in normal human tissues (fibroblasts, lymphocytes, and testes are exceptions). IMP3 plays a major role in early embryogenesis involving the development of the intestine, thymus, pancreas, and kidneys. IMP3 mRNA transcript and protein have been demonstrated in high levels in pancreatic cancer tissues but not in benign lesions of the pancreas, chronic pancreatitis, or normal pancreatic tissues.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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