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Test ID: Pap Stain Gynecology
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Pap Stain Gyne
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Useful For
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The primary purpose of Pap smear using Liquid Based Cytology is to obtaining a sample of cells from the cervix (Papanicolaou smear) to detect cervical cancer, its precursors, and other abnormalities of the reproductive tract.
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Method name and description
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SurePath Liquid Based and ThinPrep Method.
Principles of the preparation of Liquid Based Cytology (LBC) slides:
1. A sample of cells is collected from the cervix in the normal way using a spatula or broom sampling device.
2. The sample is transferred into a container of preservative/ transport medium.
3. The cells are dispersed in the fluid.
4. An aliquot of the suspension is selected for processing.
5. The cells are separated by centrifugation or filtration and deposited on a slide as a thin layer / monolayer by sedimentation or the application of pressure.
6. The slides are stained , mounted ready for microscopy.
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Aliases
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Pap smear
Liquid Based Cytology
Gynecological screening
SurePath
ThinPrep
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Specimen type / Specimen volume / Specimen container
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Cervical, Vaginal or Vault smear
Specimen Volume: 10 - 20 mL prefilled vial suspension
Container/Tube: Surepath/Thinprep Collection Vial
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Collection instructions / Special Precautions / Timing of collection
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A cervical brush or broom is recommended for taking specimens for LBC .The device is rotated 5 times around the cervical os in a clockwise direction
The brush head is removed and placed into vial which contains fixative (SurePath) or is rinsed vigorously in transport medium in vial which contains fixative (Thin Prep)
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Relevant clinical information to be provided
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Cervical and vaginal smears are primarily obtained as a screening procedure for precancerous, cancerous or inflammatory conditions. Ectocervical, endocervical and vaginal pool (posterior fornix) material may be placed on a single slide. The Pap test is a screening test for cervical cancer with an inherent small false negative rate.
It is important that the clinician ordering the test must always provide the LMP (Last Menstrual Period) of the patient as this is necessary in providing correct Gynecologic interpretation.
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Storage and transport instructions
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Specimen Rejection Criteria
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Wrong container
Wrong labeling
Incomplete Clinical Data
LMP of the patient not stated
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Biological reference intervals and clinical decision values
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Report is based on visual interpretation of stained slides by the Pathologist/Cytotechnologist in accordance with the preset international standard criteria of Gynecological evaluation.
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Turnaround time / Days and times test performed / Specimen retention time
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10 working days
Routine: Sunday-Thursday 7:00am-3:00PM
2 weeks
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