|
Test ID: U Opiate Scrn
|
|
Opiate Screen Urine
|
|
|
|
Useful For
|
To screen for drug abuse and to detect drug intoxication or overdose involving opiates.
|
|
Method name and description
|
Kinetic interaction of microparticles in solution (KIMS) as measured by changes in light transmission performed on the Roche cobas c-systems. In the absence of sample drug, soluble drug conjugates bind to antibody-bound microparticles, causing the formation of particle aggregates. As the aggregation reaction proceeds in the absence of sample drug, the absorbance increases. When a urine sample contains the drug in question, this drug competes with the drug derivative conjugate for microparticle-bound antibody. Antibody bound to sample drug is no longer available to promote particle aggregation, and subsequent particle lattice formation is inhibited. The presence of sample drug diminishes the increasing absorbance in proportion to the concentration of drug in the sample. Sample drug content is determined relative to the value obtained for a known cut-off concentration of drug.
|
|
Clinical information
|
Morphine, a natural product of the opium poppy, is a narcotic analgesic used for centuries as a medicine for the relief of severe pain.Extracted from opium obtained from the poppy’s resin, morphine may, in turn, be further chemically refined to heroin (the more potent, diacetylated analog of the parent drug). These chemically similar “opiates” reduce sensitivity to physical and psychological stimuli, dulling pain, fear and anxiety. Users are usually lethargic and indifferent. Accompanying effects may include constriction of the pupils, itching, constipation, nausea, vomiting, and respiratory depression. Death by overdose, usually resulting from dose miscalculation or dose‑strength variability, is caused by respiratory failure. (Roche Cobas Opiates package insert.)
|
|
|
Specimen type / Specimen volume / Specimen container
|
Specimen Type: Random Urine
Minimum volume of 5 mL freshly voided urine in clean container or aliquoted tube.
|
|
Collection instructions / Special Precautions / Timing of collection
|
No preservative required. Urine specimens is recommended to be stored at 2‑8°C when delay in transport.
|
|
Storage and transport instructions
|
Storage: 5 days at 2-8°C
Transport: 2-25°C
|
|
Specimen Rejection Criteria
|
Leaking specimen, contaminated sample, adulterated sample.
|
|
|
Biological reference intervals and clinical decision values
|
Qualitative assay for 300 ng/mL cut-off
Negative: <300 ng/mL
Positive: >300 ng/mL
(This is a screening test; results have to be considered in the clinical setting only. Other than clinical use, it is mandatory to confirm results with a definitive method.)
|
|
Turnaround time / Days and times test performed / Specimen retention time
|
Daily (24/7)
Turn-around time:
STAT: 1 hour
Routine: One working day
Specimen Retention: 4 days
|
|
|
