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Test ID: ENA Rib-P
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ENA Rib-P
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Anti-ribosomal P protein antibodiesc
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Useful For
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When a patient presents with signs and symptoms of a connective tissue disease (CTD) and the test for antinuclear antibodies (ANA) is positive, clinical diagnosis is necessary. Additionally, evaluating the patient for Extractable Nuclear Antigens Ribosomal (ENA Ribosomal) can aid in the clinical diagnosis of neuropsychiatric symptoms in patients with lupus erythematosus (LE).
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Method name and description
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Method: Fluorescence Enzyme Immunoassay (FEIA), Uses the EliA IgG method on Phadia system.
Using EliA technology solid phase, analyte molecules can be extracted from a sample. To achieve this, a well is coated with an antigen that specifically binds to target antibodies. Following the removal of non-bound antibodies, enzyme-labeled antibodies against human antibodies (EliA IgG Conjugate) are added to form an antibody conjugate complex. This complex is then incubated and non-bound conjugate is subsequently washed away. The bound complex is then incubated with a Development Solution, and after stopping the reaction, the fluorescence in the reaction mixture is measured. A higher response value indicates the presence of a more specific antibody in the specimen.
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Reporting name
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Anti-ribosomal P protein antibodies
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Clinical information
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Antibodies against ribosomal-P (aanti-Rib-P react against acidic phosphorylated ribosomal proteins . Anti Rib-P can be detected in approx. 15 to 20% of patients with SLE. They appear highly specific for SLE; therefore, they can be used as a diagnostic marker for the disease. Furthermore, the association has been described with particular manifestations of lupus, especially with neuropsychiatric, renal, and hepatic involvements. However, results are conflicting regarding the existence of such associations, depending on different study set-up, different study population or different sensitivity of tests used for the detection of anti Rib-P.
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Aliases
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ENA Rib-P
ENA Ribsosomal-P
Ribosomal autoantibodies
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum sample.
Specimen Volume: At minimum 2 mL whole blood or 0.5 mL serum.
Container Tube: Plan tube /Serum gel (Yellow top tubes).
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Storage and transport instructions
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For sample transportation, the sample should remain at room temperature (18-25°C ) for no longer than eight hours.
Separated serum samples can be stored at 2-8°C for one week.
Separated serum samples should be frozen at below -20°C for any long-term storage.
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Specimen Rejection Criteria
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Quantity not sufficient (QNS)
Hemolysis
Lipemic
Wrong collection container
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Biological reference intervals and clinical decision values
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Anti-ENA Ribosomal antibodies:
| Interpretation of Test Results |
Reference Values (U/ml) |
| Negative |
< 7 |
| Equivocal |
7 - 10 |
| Positive |
> 10 |
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Turnaround time / Days and times test performed / Specimen retention time
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Turnaround Time: 5 working days.
Days and Times test performed: Twice per week starting from Sunday through Thursday from 7:00 to 15:00.
Specimen retention time: Approximately 7 days.
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