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Test ID: Allergen, Grass Pollens Mix gx2
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Allergen, Grass Pollens Mix gx2
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Useful For
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Qualitative measurement of the circulating IgE antibodies to a mix of specific grass pollens allergen gx2 (Bermuda grass, Rye-grass, timothy grass, Meadow grass, Johnson grass, Bahia grass) in human serum help to identify the trigger that may be contributing to the symptoms and confirm sensitization to a particular allergen.
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Method name and description
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Method: Fluorescence Enzyme Immunoassay (FEIA), Uses the ImmunoCAP method on Phadia system.
The ImmunoCAP solid phase consists of a cellulose derivative specific for allergen enclosed in a capsule. The FEIA technology is based on an extremely high total binding capacity this ensures binding of all relevant antibodies.
The allergen of interest, covalently coupled to ImmunoCAP, reacts with the specific IgE in the patient sample. After washing away non-specific IgE, enzyme labeled antibodies against IgE are added to form a complex. Following incubation, unbound enzyme-anti IgE is washed away and the bound complex is then incubated with a developing agent. After stopping the reaction, the fluorescence of the eluate is measured. The higher the response value, the more specific IgE is present in the sample.
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Reporting name
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Allergen, Grass Pollens Mix gx2
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Clinical information
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Clinical manifestations of immediate hypersensitivity (allergic) diseases are caused by the release of proinflammatory mediators (histamine, leukotrienes, and prostaglandins) from immunoglobulin E (IgE)-sensitized effector cells (mast cells and basophils) when cell-bound IgE antibodies interact with allergen.
In vitro serum testing for IgE antibodies provides an indication of the immune response to allergen(s) that may be associated with allergic disease.
IgE - Immunoglobulin E - is a type of protein called an antibody. It plays an important role in allergy reactions and is therefore often called the “allergy antibody”.
When the person is exposed to a particular substance (allergen), the immune system starts the production of IgE as an attempt to protect the body. The specific IgE antibodies remain in the body and next time will react with specific allergen on subsequent exposure.
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Aliases
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Grass Pollens Mix gx2
Grass Pollens Mix gx2 includes: (Bermuda grass g2, Rye-grass g5 , timothy grass g6, Meadow grass g8, Johnson grass g10 , Bahia grass g17)
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum sample.
Specimen Volume: at minimum 2 mL whole blood or 1 mL serum for every 5 allergen request.
Container Tube: Plain tube (Red Top Tubes) /Serum gel (Yellow top tubes)
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Storage and transport instructions
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For sample transportation, the sample should remain at room temperature (18-25°C) for no longer than eight hours.
Separated serum samples can be stored at 2-8°C for one week.
Separated serum samples should be frozen at below -20°C for any long-term storage.
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Specimen Rejection Criteria
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Quantity not sufficient (QNS)
Hemolysis
Lipemic
Wrong collection container
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Biological reference intervals and clinical decision values
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| Interpretation |
Clinical Reaction |
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Normal |
| Positive |
Abnormal |
Interpretation:
*Positive results indicate the possibility of allergic disease induced by one or more allergens present in the multi-allergen grass pollens mix (gx2).
*Negative results may rule out allergy, except in rare cases of allergic disease induced by exposure to a single allergen.
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Factors affecting test performance and result interpretation
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Testing for IgE antibodies is not useful in patients previously treated with immunotherapy to determine if residual clinical sensitivity exists, or in patients in whom the medical management does not depend upon identification of allergen specificity.
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Turnaround time / Days and times test performed / Specimen retention time
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Turnaround Time: 5 working days.
Days and Times test performed: Sunday through Thursday from 7:00 to 15:00.
Specimen retention time: Approximately 7 days.
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