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Test ID: Reticulin Stain
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Reticulin Stain
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Useful For
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The demonstration of reticular fibers in tissue sections to differentiate diagnosis of certain types of tumors.
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Method name and description
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Gordon & Sweet Stain. The tissue is first oxidized by potassium permanganate to enhance subsequent staining of reticular fibers, and excess permanganate is removed by oxalic acid. Ferric ammonium sulfate acts as the sensitizer and is subsequently replaced by silver fro the diamine silver solution. After impregnation, formalin is used, to reduce the silver to its metallic form. Before toning with gold chloride, unreacted silver is removed with sodium thiosulfate.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Any well-fixed tissue in 10% neutral buffered formalin.
Specimen volume/ Specimen container: Submit 3-4 µm paraffin embedded tissue section mounted on a clean glass slide.
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Storage and transport instructions
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- Slides are stored in slide box at room temperature.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/Paraffin blocks mismatch
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Factors affecting test performance and result interpretation
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- Specimen fixation. Well-fixed specimens can produce consistent chemical and physical characteristics in tissue sections which allows patterns to be observed, morphological and chemical changes to be noted and comparisons made. These observations allow view of a dynamic ever-changing environment “fixed” at a particular point in time and may enable a histopathological diagnosis.
- Paraffin section thickness. Microscopic analysis of cells and tissues requires the preparation of very thin, high quality sections (3-4 µm) mounted on a clean glass slides and appropriately stained to demonstrate normal and abnormal structures.
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Turnaround time / Days and times test performed / Specimen retention time
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Turnaround time: 3 hours
Days and times test performed: Once daily (Sunday to Thursday @0700-1500)
Note: Request received after 1100H will be run/stained the following working day.
Specimen retention time: 10 years
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