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Test ID: Hematoxylin and Eosin Stain
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Hematoxylin and Eosin Stain
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Useful For
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The hematoxylin and eosin stain is the primary diagnostic stain in the practice of Anatomic pathology. H&E staining permits assessment of the general relationship among cells, allowing the basic architectural elements of epithelial, connective, nervous, and muscle tissues to be demonstrated.
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Method name and description
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Hematoxylin and Eosin stain. Hematoxylin component stains the cell nuclei blue-black with good intranuclear detail, while the eosin stains cell cytoplasm and most connective tissue fibers in varying shades and intensities of pink, orange, and red.
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Reporting name
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Hematoxylin and Eosin stain
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Clinical information
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Basic histopathology stain used for microscopic examination of all kinds of specimen.
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Specimen type / Specimen volume / Specimen container
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Specimen type: Any well-fixed tissue in 10% neutral buffered formalin.
Specimen volume/ Specimen container: Submit 3-4 µm paraffin embedded tissue section mounted on a clean glass slide.
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Collection instructions / Special Precautions / Timing of collection
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Storage and transport instructions
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- Slides are stored in slide box at room temperature.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/Paraffin blocks mismatch
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Biological reference intervals and clinical decision values
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Factors affecting test performance and result interpretation
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- Specimen fixation. Well-fixed specimens can produce consistent chemical and physical characteristics in tissue sections which allows patterns to be observed, morphological and chemical changes to be noted and comparisons made. These observations allow view of a dynamic ever-changing environment “fixed” at a particular point in time and may enable a histopathological diagnosis.
- Paraffin section thickness. Microscopic analysis of cells and tissues requires the preparation of very thin, high quality sections (3-4 µm) mounted on a clean glass slides and appropriately stained to demonstrate normal and abnormal structures.
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Turnaround time / Days and times test performed / Specimen retention time
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Turnaround time: 2 hours
Days and times test performed: Continous staining in a day (Sunday to Thursday @0700-1500)
Note: Request received after 1300H will be run/stained the following working day.
Specimen retention time: 10 years
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