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Test ID: Adenovirus PCR
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Adenovirus (ADV) PCR
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Useful For
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In vitro diagnostic test for the detection and quantification of Adenovirus specific DNA in human EDTA plasma
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Method name and description
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Commercial in vitro diagnostic test based on real-time PCR technology, for the detection and quantification of human adenovirus (HAdV) specific DNA.
Real-time PCR technology utilizes polymerase chain reaction (PCR) for the amplification of specific target sequences and target specific probes for the detection of the amplified DNA. The probes are labelled with fluorescent reporter and quencher dyes.
Using probes linked to distinguishable dyes enables the parallel detection of HAdV specific DNA and the Internal Control in corresponding detector channels of the real-time PCR instrument. The Quantification Standards contain standardised concentrations of HAdV specific DNA (Manufacturer package insert)
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Clinical information
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Adenoviruses are ubiquitous in the environment where contamination by human faeces or sewage has occurred. Adenoviruses most commonly cause respiratory illness. The illnesses can range from the common cold to pneumonia, croup, and bronchitis. Depending on the type, adenoviruses can cause other illnesses such as gastroenteritis, conjunctivitis, cystitis, and, less commonly, neurological disease. People with weakened immune systems are at high risk for developing severe illness caused by adenovirus infection.
https://www.cdc.gov/adenovirus/hcp/clinical-overview.html
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Aliases
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ACE PCR
Human adenoviruses (HAdV) PCR
ADV PCR
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Specimen type / Specimen volume / Specimen container
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Specimen type: Plasma
Specimen Volume: 2-5 mL
Specimen container/Tube: Whole blood in EDTA tube
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Collection instructions / Special Precautions / Timing of collection
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Collection by venipuncture as per organization guidelines
No special precautions / Timing of collection
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Relevant clinical information to be provided
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Description of symptoms and history of the clinical presentation
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Storage and transport instructions
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The specimens should be transported immediately to Central Processing (CP) in the Qatar Rehabilitation Institute (QRI), 3rd floor, HBK MC in cool box with ice pack.
If blood specimen(s) cannot be delivered immediately to CP, store in a refrigerator (4 - 8◦C) for no more than 6 hours. Beyond that, separate plasma (centrifugation) and store at -70◦C
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Specimen Rejection Criteria
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- An inadequate volume of specimen for testing is received (QNS).
- The specimen has leaked
- Heparinized blood
- The specimen is in a wrong container
- Improper storage of specimen before submission to virology laboratory.
- Clots detected in plasma by receiving bench or by instruments
- Patient information on request does not match with information on the sample tube/container.
- There is gross hemolysis in plasma sample
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Biological reference intervals and clinical decision values
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- Positive: ADV specific DNA detected.
- Negative: No ADV specific DNA detected. Sample does not contain detectable amounts of ADV specific DNA
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Turnaround time / Days and times test performed / Specimen retention time
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Turnaround time: within 7 days
Test performed twice a week
Specimen retention time: 1 month
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