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Test ID: Rotavirus/Adeno Detection Stool
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Adenovirus and Rotavirus antigens in stool
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Detection of adenovirus and rotavirus antigens in stool in cases of suspected viral gastroenteritis
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Useful For
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Detection of Rotavirus and Adenovirus in human stools in suspected viral gastroenteritis
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Method name and description
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Commercially available Immuno-chromatographic assay
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Reporting name
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Rotavirus/Adeno Detection Stool
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Clinical information
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Rotavirus is the most common cause of viral severe diarrhea among infants and young children and is one of several viruses that can cause diarrheal infections often called stomach flu, which is unrelated to influenza influenza.
Adenovirus infections most commonly cause diseases of the upper respiratory tract; however, depending on the infective serotype, they may also cause various other illnesses, such as gastroenteritis, conjunctivitis, cystitis, and rashes.
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Aliases
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- Rotavirus in stool
- Adenovirus in stool
- Rotavirus/Adenovirus in stool
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Specimen type / Specimen volume / Specimen container
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Specimen type
Specimen volume
- 1-2 ml or 10-20 g of stool
Specimen container
- Clean leak proof wide mouth container, containing no detergents, preservatives or transport media.
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Collection instructions / Special Precautions / Timing of collection
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Collection instructions
Patients should be instructed to collect the stool in the container, taking care to avoid mixing with urine or toilet water
Timing of collection
Collection at the onset of the symptoms is preferable.
Viral detection is improved by collecting the specimens at the onset of the symptoms. It has been reported that the maximum excretion of rotavirus and adenovirus in the stools of patients with gastroenteritis occurs 3-5 days and 3-13 days respectively after the symptoms have appeared.
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Relevant clinical information to be provided
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- Date of onset of symptoms
- If part of an outbreak
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Storage and transport instructions
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- ≤2 hrs. at room temperature
- Specimen should be stored at 2-8°C if any delay and transported to the laboratory within 24 hrs of collection
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Specimen Rejection Criteria
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- Leaking container
- Unlabeled/Mislabeled specimen
- Improper container
- Duplicate samples within 72 hrs
- Quantity not sufficient
- No relevant clinical details provided with the request. (Please note that where applicable, the relevant clinical details should reflect those on the patient’s electronic record at the time the order was placed).
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Biological reference intervals and clinical decision values
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Negative report
- Adenovirus: Negative
- Rotavirus: Negative
Positive report
- Rotavirus: Positive and/or
- Adenovirus: Positive
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Factors affecting test performance and result interpretation
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If the samples are collected long after the onset of diarrheal symptoms, the quantity of antigen may not be sufficient to obtain a positive reaction or the antigens detected may not be linked to the diarrheal episode.
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Turnaround time / Days and times test performed / Specimen retention time
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Turnaround time
Days and times test performed
- Sunday to Thursday 7am-3 pm at HGH microbiology laboratory; 24/7 at AAH, AWH and TCH microbiology laboratories
Specimen retention time
- 48 hrs. after final report
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