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Test ID: Entamoeba histolytica Antibody
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Entamoeba histolytica Antibody
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Useful For
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Semi quantitative determination of specific antibodies to Entamoeba histolytica in human serum
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Method name and description
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Indirect hemagglutination test.
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Reporting name
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Entamoeba histolytica Antibody
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Aliases
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E. Histolytica Antibody, Entamoeba; Amoebic Serology; Amebiasis
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Specimen type / Specimen volume / Specimen container
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Specimen type: Serum
Specimen Volume: 3-5 mL
Specimen container/Tube: Serum gel (Yellow top tubes)
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Collection instructions / Special Precautions / Timing of collection
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Collection by venipuncture as per organization guidelines
No special precautions / no Timing of collection
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Storage and transport instructions
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Maintain specimen at 2-8°C
The specimens should be transported to Virology Section in cold box immediately
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Specimen Rejection Criteria
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Quantity of sample not sufficient
lipemic sample
Hemolysed sample
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Biological reference intervals and clinical decision values
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1- Titer < 1:80 Negative (Considered Negative)
2- 1:80 ≤ Titer ≤1:160 Non significant reaction
3-Titer ≥ 1:320 Significant reaction in favour of visceral amoebiasis.
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Factors affecting test performance and result interpretation
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Certain sera, whose antibody concentration is very high, can give rise to a prozone phenomenon (with disappearance of the clouding) in the initial dilutions, which disappears in the subsequent dilutions.
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Turnaround time / Days and times test performed / Specimen retention time
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Turnaround time: within 7 days
Days and times test performed: Twice a week
Specimen retention time: 2 weeks
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