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Test ID: IH CA125
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Cancer Antigen 125
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Immunohistochemistry Stain
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Useful For
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Identification of serous ovarian carcinoma, but nonspecific. Antibodies to CA 125 may also be useful for the identification of mesothelioma, but nonspecific.
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Method name and description
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- Monoclonal Mouse Anti-Human CA 125, Clone M11
- Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Clinical information
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CA 125 is a large type 1 transmembrane glycoprotein consisting of more than 22000 amino acids with a small intracellular and a large extracellular domain. It is encoded by the MUC16 gene mapped to 19p13.3 by sequence analysis. CA 125 is secreted by the endoplasmic reticulum and golgi apparatus by a mechanism signal independent. The structure of CA 125 has been well elucidated, but there is little known about the function of the protein, although it appears to be a part of a high molecular weight mucin-like glycoprotein complex. CA 125 is expressed on the cell surface in embryonic membranes and adult derivatives of fetal periderm. The antigen is expressed by ovarian tumors and can also be found in the sera of patients with ovarian, pancreatic, liver, colon adenocarcinoma.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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