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Test ID: IH E-Cad
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E-Cadherin
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Immunohistochemistry Stain
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Useful For
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Differentiated diagnosis between ductal carcinoma and lobular carcinoma of the breast.
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Method name and description
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- Monoclonal Mouse Anti-Human E-cadherin, Clone NCH-38.
- Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Clinical information
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E-cadherin is a 120kDa transmembrane cell adhesion molecule. The gene has been localized on chromosome 16q221. In its extracellular domain, E-cadherin is involved in cell-cell adhesion through calcium-regulated homophilic interactions, whereas in its intracellular domain, E-cadherin connects to the actin cytoskeleton via catenins. E-cadherin has a significant function in intracellular adhesion of epithelial cells, the establishment of epithelial polarization, glandular differentiation, and stratification. It is localized mainly in the adherens junctions and concentrates the urokinase plasminogen activator and the epidermal growth factor receptor to cell contract sites. Down-regulation of E-cadherin expression has been observed in a number of carcinomas and is usually associated with advance stage and progression.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide e (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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