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Test ID: IH Oct3/4
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Octamer-Binding Transcription Factor 3/4
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Immunohistochemistry Stain
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Useful For
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Identification of specific subtypes of germ cell tumors including seminoma, embryonal carcinoma, and intratubular germ cell neoplasia of unclassified type (IGCNU). Negative in Yolk Sac tumors.
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Method name and description
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- Monoclonal Mouse Anti-Human Octamer-Binding Transcription Factor 3/4, Clone N1NK.
- Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Clinical information
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Alternative splicing gives rise to two mRNA variants from the human OCT4 gene on chromosome 6, originally named OCT3A and OCT3B, thus the adapted OCT3/4 name. The OCT3A and OCT3B isoforms are composed of 360 and 265 amino acids, respectively, of which the 225 amino acids at the C-termini are identical. OCT3/4 belongs to the POU (Pit-Oct-Unc) transcription factor family which can activate the expression of their target genes through binding an octameric sequence motif of an AGTCAAAT consensus sequence. OCT3/4 is expressed in early embryonic cells and germ cells and is central to the gene regulatory network responsible for self-renewal, pluripotency, and lineage commitment in embryonic stem cells and induced pluripotent stem cells. OCT3/4 expression is also detected in specific subtypes of germ cell tumors, such as seminomas, germinomas, dysgerminoma, embryonal carcinoma, the germ cell component of gonadoblastomas, and IGCNU.
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Aliases
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Octamer-Binding Transcription Factor 3/4.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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