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Test ID: IH p16
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p16
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Immunohistochemistry Stain
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Useful For
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Expressed in HPV infected tissue (High grade CIN, Cervical carcinomas and oropharyngeal carcinoma).
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Method name and description
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- Mouse Monoclonal Anti-p16, Clone E6H4.
- Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Clinical information
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As a cyclin-dependent kinase inhibitor, p16 plays a key role in cell cycle progression and cellular differentiation. The p16 protein is part of the retinoblastoma protein(pRB)-meditated control of the G1-S-phase transition and triggers cell cycle arrest during the cellular differentiation process. In normal, terminally differentiated cells, p16 is expressed at low levels, typically not detectable by immunohistochemistry. However, in some tumor entities, overexpression of p16 protein has been demonstrated to contribute to cell cycle deregulation and cellular transformation.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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