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Test ID: IH P40(P)
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P40(P)
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Immunohistochemistry Stain
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Useful For
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P40 may prove to be an important antibody in the differential diagnosis of lung adenocarcinoma versus lung squamous cell carcinoma.
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Method name and description
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- Rabbit polyclonal P40 (P) Antibody.
- Immunoperoxidase stain on formalin-fixed, paraffin-embedded (FFPE) tissue section
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Clinical information
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p40 recognizes the shortest variant of human p53 and may be a valuable marker in cases where p63 has traditionally been used. At present, p63 is the most frequently used marker for lung squamous cell carcinoma (SqCC). p63 is extremely sensitive for SqCC; however, it suffers from specificity limitations due to its reactivity in a subset of lung adenocarcinomas. Diagnosing non-small cell lung cancer (NSCLC) by morphology in small samples (e.g. biopsy, cellblock, FNA) can be difficult. Given that conserving tissue for molecular testing is a priority, a minimalist immunohistochemistry (IHC)-based diagnostic approach is warranted.
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Specimen type / Specimen volume / Specimen container
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- Specimen type: Well-fixed tissue in 10% neutral buffered formalin.
- Submit 2 unstained (3-4 µm thick) paraffin embedded tissue section mounted on a clean positively charged glass slide (sections to be cut within 6 weeks.) or formalin-fixed, paraffin-embedded (FFPE) tissue block.
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Storage and transport instructions
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- Slides or blocks are stored in cork box at room temperature away from sun light and any source of heat.
- Follow your local regulation shipping guidelines.
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Specimen Rejection Criteria
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- Broken slides
- Unlabeled slides with patient/case identification
- Contaminated slides
- Slides/paraffin blocks mismatch
- Uncharged slides
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Factors affecting test performance and result interpretation
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- Fixation time (FT)
- Fixative Type
- Storage time in paraffin
- Storage temperature
- Age of the cut sections
- Section thickness
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Turnaround time / Days and times test performed / Specimen retention time
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- Turnaround time for the test in platform: 3-4 hours
- Days and times test performed: Twice daily (Sunday to Wednesday)
- First batch: 0600H-1400H
- Second batch; 1230H - overnight
- Thursday (one batch only): 0600H-1400H
- Note: Request received after 1230H will be stained the following working day.
- Shelf-life of the paraffin section slides: ≤6 weeks
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